Evidence for Nanoparticle-Induced Lysosomal Dysfunction in Lung Adenocarcinoma (A549) Cells
Identifieur interne : 000826 ( Main/Exploration ); précédent : 000825; suivant : 000827Evidence for Nanoparticle-Induced Lysosomal Dysfunction in Lung Adenocarcinoma (A549) Cells
Auteurs : Arnold Sipos [États-Unis] ; Kwang-Jin Kim [États-Unis] ; Constantinos Sioutas ; Edward D. Crandall [États-Unis]Source :
- International Journal of Molecular Sciences [ 1422-0067 ] ; 2019.
Abstract
Background: Polystyrene nanoparticles (PNP) are taken up by primary rat alveolar epithelial cell monolayers (RAECM) in a time-, dose-, and size-dependent manner without involving endocytosis. Internalized PNP in RAECM activate autophagy, are delivered to lysosomes, and undergo [Ca2+]-dependent exocytosis. In this study, we explored nanoparticle (NP) interactions with A549 cells. Methods: After exposure to PNP or ambient pollution particles (PM0.2), live single A549 cells were studied using confocal laser scanning microscopy. PNP uptake and egress were investigated and activation of autophagy was confirmed by immunolabeling with LC3-II and LC3-GFP transduction/colocalization with PNP. Mitochondrial membrane potential, mitophagy, and lysosomal membrane permeability (LMP) were assessed in the presence/absence of apical nanoparticle (NP) exposure. Results: PNP uptake into A549 cells decreased in the presence of cytochalasin D, an inhibitor of macropinocytosis. PNP egress was not affected by increased cytosolic [Ca2+]. Autophagy activation was indicated by increased LC3 expression and LC3-GFP colocalization with PNP. Increased LMP was observed following PNP or PM0.2 exposure. Mitochondrial membrane potential was unchanged and mitophagy was not detected after NP exposure. Conclusions: Interactions between NP and A549 cells involve complex cellular processes leading to lysosomal dysfunction, which may provide opportunities for improved nanoparticle-based therapeutic approaches to lung cancer management.
Url:
DOI: 10.3390/ijms20215253
PubMed: 31652767
PubMed Central: 6861930
Affiliations:
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(E.D.C.)</nlm:aff></affiliation><affiliation wicri:level="4"><nlm:aff id="af2-ijms-20-05253">Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-0906, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-0906</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Kim, Kwang Jin" sort="Kim, Kwang Jin" uniqKey="Kim K" first="Kwang-Jin" last="Kim">Kwang-Jin Kim</name><affiliation><nlm:aff id="af1-ijms-20-05253">Will Rogers Institute Pulmonary Research Center and Hastings Center for Pulmonary Research, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-0906, USA;<email>kjkim@usc.edu</email> (K.-J.K.);<email>edward.crandall@med.usc.edu</email> (E.D.C.)</nlm:aff></affiliation><affiliation wicri:level="4"><nlm:aff id="af2-ijms-20-05253">Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-0906, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-0906</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="af3-ijms-20-05253">Department of Physiology and Neuroscience, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089-9037, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physiology and Neuroscience, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089-9037</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="af4-ijms-20-05253">Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089-9121, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089-9121</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="af5-ijms-20-05253">Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089-1111, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089-1111</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Sioutas, Constantinos" sort="Sioutas, Constantinos" uniqKey="Sioutas C" first="Constantinos" last="Sioutas">Constantinos Sioutas</name><affiliation><nlm:aff id="af6-ijms-20-05253">Sonny Astani Department of Civil and Environmental Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089-2531, USA;<email>sioutas@usc.edu</email></nlm:aff></affiliation></author><author><name sortKey="Crandall, Edward D" sort="Crandall, Edward D" uniqKey="Crandall E" first="Edward D." last="Crandall">Edward D. Crandall</name><affiliation><nlm:aff id="af1-ijms-20-05253">Will Rogers Institute Pulmonary Research Center and Hastings Center for Pulmonary Research, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-0906, USA;<email>kjkim@usc.edu</email> (K.-J.K.);<email>edward.crandall@med.usc.edu</email> (E.D.C.)</nlm:aff></affiliation><affiliation wicri:level="4"><nlm:aff id="af2-ijms-20-05253">Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-0906, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-0906</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="af7-ijms-20-05253">Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-9092, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033-9092</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="af8-ijms-20-05253">Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089-1211, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089-1211</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">International Journal of Molecular Sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Background: Polystyrene nanoparticles (PNP) are taken up by primary rat alveolar epithelial cell monolayers (RAECM) in a time-, dose-, and size-dependent manner without involving endocytosis. Internalized PNP in RAECM activate autophagy, are delivered to lysosomes, and undergo [Ca<sup>2+</sup>]-dependent exocytosis. In this study, we explored nanoparticle (NP) interactions with A549 cells. Methods: After exposure to PNP or ambient pollution particles (PM0.2), live single A549 cells were studied using confocal laser scanning microscopy. PNP uptake and egress were investigated and activation of autophagy was confirmed by immunolabeling with LC3-II and LC3-GFP transduction/colocalization with PNP. Mitochondrial membrane potential, mitophagy, and lysosomal membrane permeability (LMP) were assessed in the presence/absence of apical nanoparticle (NP) exposure. Results: PNP uptake into A549 cells decreased in the presence of cytochalasin D, an inhibitor of macropinocytosis. PNP egress was not affected by increased cytosolic [Ca<sup>2+</sup>]. Autophagy activation was indicated by increased LC3 expression and LC3-GFP colocalization with PNP. Increased LMP was observed following PNP or PM0.2 exposure. Mitochondrial membrane potential was unchanged and mitophagy was not detected after NP exposure. Conclusions: Interactions between NP and A549 cells involve complex cellular processes leading to lysosomal dysfunction, which may provide opportunities for improved nanoparticle-based therapeutic approaches to lung cancer management.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Buzea, C" uniqKey="Buzea C">C. Buzea</name></author><author><name sortKey="Pacheco, I I" uniqKey="Pacheco I">I.I. Pacheco</name></author><author><name sortKey="Robbie, K" uniqKey="Robbie K">K. Robbie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Khan, M I" uniqKey="Khan M">M.I. Khan</name></author><author><name sortKey="Mohammad, A" uniqKey="Mohammad A">A. Mohammad</name></author><author><name sortKey="Patil, G" uniqKey="Patil G">G. Patil</name></author><author><name sortKey="Naqvi, S A H" uniqKey="Naqvi S">S.A.H. Naqvi</name></author><author><name sortKey="Chauhan, L K S" uniqKey="Chauhan L">L.K.S. Chauhan</name></author><author><name sortKey="Ahmad, I" uniqKey="Ahmad I">I. Ahmad</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Panariti, A" uniqKey="Panariti A">A. Panariti</name></author><author><name sortKey="Miserocchi, G" uniqKey="Miserocchi G">G. Miserocchi</name></author><author><name sortKey="Rivolta, I" uniqKey="Rivolta I">I. Rivolta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cao, Y" uniqKey="Cao Y">Y. Cao</name></author><author><name sortKey="Long, J" uniqKey="Long J">J. Long</name></author><author><name sortKey="Liu, L" uniqKey="Liu L">L. Liu</name></author><author><name sortKey="He, T" uniqKey="He T">T. He</name></author><author><name sortKey="Jiang, L" uniqKey="Jiang L">L. Jiang</name></author><author><name sortKey="Zhao, C" uniqKey="Zhao C">C. Zhao</name></author><author><name sortKey="Li, Z" uniqKey="Li Z">Z. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Andersson, P O" uniqKey="Andersson P">P.O. Andersson</name></author><author><name sortKey="Lejon, C" uniqKey="Lejon C">C. Lejon</name></author><author><name sortKey="Ekstrand Hammarstrom, B" uniqKey="Ekstrand Hammarstrom B">B. Ekstrand-Hammarström</name></author><author><name sortKey="Akfur, C" uniqKey="Akfur C">C. Akfur</name></author><author><name sortKey="Ahlinder, L" uniqKey="Ahlinder L">L. Ahlinder</name></author><author><name sortKey="Bucht, A" uniqKey="Bucht A">A. Bucht</name></author><author><name sortKey="Osterlund, L" uniqKey="Osterlund L">L. Österlund</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bussy, C" uniqKey="Bussy C">C. Bussy</name></author><author><name sortKey="Pinault, M" uniqKey="Pinault M">M. Pinault</name></author><author><name sortKey="Cambedouzou, J" uniqKey="Cambedouzou J">J. Cambedouzou</name></author><author><name sortKey="Landry, M J" uniqKey="Landry M">M.J. Landry</name></author><author><name sortKey="Jegou, P" uniqKey="Jegou P">P. Jegou</name></author><author><name sortKey="Mayne L Ermite, M" uniqKey="Mayne L Ermite M">M. Mayne-L’hermite</name></author><author><name sortKey="Launois, P" uniqKey="Launois P">P. Launois</name></author><author><name sortKey="Boczkowski, J" uniqKey="Boczkowski J">J. Boczkowski</name></author><author><name sortKey="Lanone, S" uniqKey="Lanone S">S. Lanone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gazzano, E" uniqKey="Gazzano E">E. Gazzano</name></author><author><name sortKey="Ghiazza, M" uniqKey="Ghiazza M">M. Ghiazza</name></author><author><name sortKey="Polimeni, M" uniqKey="Polimeni M">M. Polimeni</name></author><author><name sortKey="Bolis, V" uniqKey="Bolis V">V. Bolis</name></author><author><name sortKey="Fenoglio, I" uniqKey="Fenoglio I">I. Fenoglio</name></author><author><name sortKey="Attanasio, A" uniqKey="Attanasio A">A. Attanasio</name></author><author><name sortKey="Mazzucco, G" uniqKey="Mazzucco G">G. Mazzucco</name></author><author><name sortKey="Fubini, B" uniqKey="Fubini B">B. Fubini</name></author><author><name sortKey="Ghigo, D" uniqKey="Ghigo D">D. Ghigo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chusuei, C C" uniqKey="Chusuei C">C.C. Chusuei</name></author><author><name sortKey="Wu, C H" uniqKey="Wu C">C.-H. Wu</name></author><author><name sortKey="Mallavarapu, S" uniqKey="Mallavarapu S">S. Mallavarapu</name></author><author><name sortKey="Hou, F Y S" uniqKey="Hou F">F.Y.S. Hou</name></author><author><name sortKey="Hsu, C M" uniqKey="Hsu C">C.-M. Hsu</name></author><author><name sortKey="Winiarz, J G" uniqKey="Winiarz J">J.G. Winiarz</name></author><author><name sortKey="Aronstam, R S" uniqKey="Aronstam R">R.S. Aronstam</name></author><author><name sortKey="Huang, Y W" uniqKey="Huang Y">Y.-W. Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kang, S" uniqKey="Kang S">S. Kang</name></author><author><name sortKey="Kim, J E" uniqKey="Kim J">J.-E. Kim</name></author><author><name sortKey="Kim, D" uniqKey="Kim D">D. Kim</name></author><author><name sortKey="Woo, C G" uniqKey="Woo C">C.G. Woo</name></author><author><name sortKey="Pikhitsa, P V" uniqKey="Pikhitsa P">P.V. Pikhitsa</name></author><author><name sortKey="Cho, M H" uniqKey="Cho M">M.-H. Cho</name></author><author><name sortKey="Choi, M" uniqKey="Choi M">M. Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, K N" uniqKey="Yu K">K.-N. Yu</name></author><author><name sortKey="Yoon, T J" uniqKey="Yoon T">T.-J. Yoon</name></author><author><name sortKey="Minai Tehrani, A" uniqKey="Minai Tehrani A">A. Minai-Tehrani</name></author><author><name sortKey="Kim, J E" uniqKey="Kim J">J.-E. Kim</name></author><author><name sortKey="Park, S J" uniqKey="Park S">S.J. Park</name></author><author><name sortKey="Jeong, M S" uniqKey="Jeong M">M.S. Jeong</name></author><author><name sortKey="Ha, S W" uniqKey="Ha S">S.-W. Ha</name></author><author><name sortKey="Lee, J K" uniqKey="Lee J">J.-K. Lee</name></author><author><name sortKey="Kim, J S" uniqKey="Kim J">J.S. Kim</name></author><author><name sortKey="Cho, M H" uniqKey="Cho M">M.-H. Cho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, C" uniqKey="Guo C">C. Guo</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Jing, L" uniqKey="Jing L">L. Jing</name></author><author><name sortKey="Ma, R" uniqKey="Ma R">R. Ma</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X. Liu</name></author><author><name sortKey="Gao, L" uniqKey="Gao L">L. Gao</name></author><author><name sortKey="Cao, L" uniqKey="Cao L">L. Cao</name></author><author><name sortKey="Duan, J" uniqKey="Duan J">J. Duan</name></author><author><name sortKey="Zhou, X" uniqKey="Zhou X">X. Zhou</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, K N" uniqKey="Yu K">K.-N. Yu</name></author><author><name sortKey="Chang, S H" uniqKey="Chang S">S.-H. Chang</name></author><author><name sortKey="Park, S J" uniqKey="Park S">S.J. Park</name></author><author><name sortKey="Lim, J" uniqKey="Lim J">J. Lim</name></author><author><name sortKey="Lee, J" uniqKey="Lee J">J. Lee</name></author><author><name sortKey="Yoon, T J" uniqKey="Yoon T">T.-J. Yoon</name></author><author><name sortKey="Kim, J S" uniqKey="Kim J">J.-S. Kim</name></author><author><name sortKey="Cho, M H" uniqKey="Cho M">M.-H. Cho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tooze, S A" uniqKey="Tooze S">S.A. Tooze</name></author><author><name sortKey="Abada, A" uniqKey="Abada A">A. Abada</name></author><author><name sortKey="Elazar, Z" uniqKey="Elazar Z">Z. Elazar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Glick, D" uniqKey="Glick D">D. Glick</name></author><author><name sortKey="Barth, S" uniqKey="Barth S">S. Barth</name></author><author><name sortKey="Macleod, K F" uniqKey="Macleod K">K.F. Macleod</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dikic, I" uniqKey="Dikic I">I. Dikic</name></author><author><name sortKey="Elazar, Z" uniqKey="Elazar Z">Z. Elazar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Codogno, P" uniqKey="Codogno P">P. Codogno</name></author><author><name sortKey="Meijer, A J" uniqKey="Meijer A">A.J. Meijer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Das, G" uniqKey="Das G">G. Das</name></author><author><name sortKey="Shravage, B V" uniqKey="Shravage B">B.V. Shravage</name></author><author><name sortKey="Baehrecke, E H" uniqKey="Baehrecke E">E.H. Baehrecke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denton, D" uniqKey="Denton D">D. Denton</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S. Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Klionsky, D J" uniqKey="Klionsky D">D.J. Klionsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Klionsky, D J" uniqKey="Klionsky D">D.J. Klionsky</name></author><author><name sortKey="Eskelinen, E L" uniqKey="Eskelinen E">E.-L. Eskelinen</name></author><author><name sortKey="Deretic, V" uniqKey="Deretic V">V. Deretic</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Manshian, B B" uniqKey="Manshian B">B.B. Manshian</name></author><author><name sortKey="Pokhrel, S" uniqKey="Pokhrel S">S. Pokhrel</name></author><author><name sortKey="M Dler, L" uniqKey="M Dler L">L. Mädler</name></author><author><name sortKey="Soenen, S J" uniqKey="Soenen S">S.J. Soenen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Conti, S" uniqKey="Conti S">S. Conti</name></author><author><name sortKey="Harari, S" uniqKey="Harari S">S. Harari</name></author><author><name sortKey="Caminati, A" uniqKey="Caminati A">A. Caminati</name></author><author><name sortKey="Zanobetti, A" uniqKey="Zanobetti A">A. Zanobetti</name></author><author><name sortKey="Schwartz, J D" uniqKey="Schwartz J">J.D. Schwartz</name></author><author><name sortKey="Bertazzi, P A" uniqKey="Bertazzi P">P.A. Bertazzi</name></author><author><name sortKey="Cesana, G" uniqKey="Cesana G">G. Cesana</name></author><author><name sortKey="Madotto, F" uniqKey="Madotto F">F. Madotto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kurt, O K" uniqKey="Kurt O">O.K. Kurt</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J. Zhang</name></author><author><name sortKey="Pinkerton, K E" uniqKey="Pinkerton K">K.E. Pinkerton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, D" uniqKey="Kim D">D. Kim</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Zhou, L F" uniqKey="Zhou L">L.-F. Zhou</name></author><author><name sortKey="Huang, S X" uniqKey="Huang S">S.-X. Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nardone, L L" uniqKey="Nardone L">L.L. Nardone</name></author><author><name sortKey="Andrews, S B" uniqKey="Andrews S">S.B. Andrews</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sipos, A" uniqKey="Sipos A">A. Sipos</name></author><author><name sortKey="Kim, K J" uniqKey="Kim K">K.-J. Kim</name></author><author><name sortKey="Chow, R H" uniqKey="Chow R">R.H. Chow</name></author><author><name sortKey="Flodby, P" uniqKey="Flodby P">P. Flodby</name></author><author><name sortKey="Borok, Z" uniqKey="Borok Z">Z. Borok</name></author><author><name sortKey="Crandall, E D" uniqKey="Crandall E">E.D. Crandall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Contini, C" uniqKey="Contini C">C. Contini</name></author><author><name sortKey="Schneemilch, M" uniqKey="Schneemilch M">M. Schneemilch</name></author><author><name sortKey="Gaisford, S" uniqKey="Gaisford S">S. Gaisford</name></author><author><name sortKey="Quirke, N" uniqKey="Quirke N">N. Quirke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Behzadi, S" uniqKey="Behzadi S">S. Behzadi</name></author><author><name sortKey="Serpooshan, V" uniqKey="Serpooshan V">V. Serpooshan</name></author><author><name sortKey="Tao, W" uniqKey="Tao W">W. Tao</name></author><author><name sortKey="Hamaly, M A" uniqKey="Hamaly M">M.A. Hamaly</name></author><author><name sortKey="Alkawareek, M Y" uniqKey="Alkawareek M">M.Y. Alkawareek</name></author><author><name sortKey="Dreaden, E C" uniqKey="Dreaden E">E.C. Dreaden</name></author><author><name sortKey="Brown, D" uniqKey="Brown D">D. Brown</name></author><author><name sortKey="Alkilany, A M" uniqKey="Alkilany A">A.M. Alkilany</name></author><author><name sortKey="Farokhzad, O C" uniqKey="Farokhzad O">O.C. Farokhzad</name></author><author><name sortKey="Mahmoudi, M" uniqKey="Mahmoudi M">M. Mahmoudi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Treuel, L" uniqKey="Treuel L">L. Treuel</name></author><author><name sortKey="Jiang, X" uniqKey="Jiang X">X. Jiang</name></author><author><name sortKey="Nienhaus, G U" uniqKey="Nienhaus G">G.U. Nienhaus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodriguez, A" uniqKey="Rodriguez A">A. Rodríguez</name></author><author><name sortKey="Webster, P" uniqKey="Webster P">P. Webster</name></author><author><name sortKey="Ortego, J" uniqKey="Ortego J">J. Ortego</name></author><author><name sortKey="Andrews, N W" uniqKey="Andrews N">N.W. Andrews</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, J" uniqKey="Xu J">J. Xu</name></author><author><name sortKey="Toops, K A" uniqKey="Toops K">K.A. Toops</name></author><author><name sortKey="Diaz, F" uniqKey="Diaz F">F. Diaz</name></author><author><name sortKey="Carvajal Gonzalez, J M" uniqKey="Carvajal Gonzalez J">J.M. Carvajal-Gonzalez</name></author><author><name sortKey="Gravotta, D" uniqKey="Gravotta D">D. Gravotta</name></author><author><name sortKey="Mazzoni, F" uniqKey="Mazzoni F">F. Mazzoni</name></author><author><name sortKey="Schreiner, R" uniqKey="Schreiner R">R. Schreiner</name></author><author><name sortKey="Rodriguez Boulan, E" uniqKey="Rodriguez Boulan E">E. Rodriguez-Boulan</name></author><author><name sortKey="Lakkaraju, A" uniqKey="Lakkaraju A">A. Lakkaraju</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kabeya, Y" uniqKey="Kabeya Y">Y. Kabeya</name></author><author><name sortKey="Mizushima, N" uniqKey="Mizushima N">N. Mizushima</name></author><author><name sortKey="Ueno, T" uniqKey="Ueno T">T. Ueno</name></author><author><name sortKey="Yamamoto, A" uniqKey="Yamamoto A">A. Yamamoto</name></author><author><name sortKey="Kirisako, T" uniqKey="Kirisako T">T. Kirisako</name></author><author><name sortKey="Noda, T" uniqKey="Noda T">T. Noda</name></author><author><name sortKey="Kominami, E" uniqKey="Kominami E">E. Kominami</name></author><author><name sortKey="Ohsumi, Y" uniqKey="Ohsumi Y">Y. Ohsumi</name></author><author><name sortKey="Yoshimori, T" uniqKey="Yoshimori T">T. Yoshimori</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kimura, S" uniqKey="Kimura S">S. Kimura</name></author><author><name sortKey="Noda, T" uniqKey="Noda T">T. Noda</name></author><author><name sortKey="Yoshimori, T" uniqKey="Yoshimori T">T. Yoshimori</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Antonioli, M" uniqKey="Antonioli M">M. Antonioli</name></author><author><name sortKey="Di Rienzo, M" uniqKey="Di Rienzo M">M. Di Rienzo</name></author><author><name sortKey="Piacentini, M" uniqKey="Piacentini M">M. Piacentini</name></author><author><name sortKey="Fimia, G M" uniqKey="Fimia G">G.M. Fimia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, Y T" uniqKey="Wu Y">Y.-T. Wu</name></author><author><name sortKey="Tan, H L" uniqKey="Tan H">H.-L. Tan</name></author><author><name sortKey="Shui, G" uniqKey="Shui G">G. Shui</name></author><author><name sortKey="Bauvy, C" uniqKey="Bauvy C">C. Bauvy</name></author><author><name sortKey="Huang, Q" uniqKey="Huang Q">Q. Huang</name></author><author><name sortKey="Wenk, M R" uniqKey="Wenk M">M.R. Wenk</name></author><author><name sortKey="Ong, C N" uniqKey="Ong C">C.-N. Ong</name></author><author><name sortKey="Codogno, P" uniqKey="Codogno P">P. Codogno</name></author><author><name sortKey="Shen, H M" uniqKey="Shen H">H.-M. Shen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="White, E" uniqKey="White E">E. White</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Folkerts, H" uniqKey="Folkerts H">H. Folkerts</name></author><author><name sortKey="Hilgendorf, S" uniqKey="Hilgendorf S">S. Hilgendorf</name></author><author><name sortKey="Vellenga, E" uniqKey="Vellenga E">E. Vellenga</name></author><author><name sortKey="Bremer, E" uniqKey="Bremer E">E. Bremer</name></author><author><name sortKey="Wiersma, V R" uniqKey="Wiersma V">V.R. Wiersma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, Y G" uniqKey="Zhao Y">Y.G. Zhao</name></author><author><name sortKey="Zhang, H" uniqKey="Zhang H">H. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pierzy Ska Mach, A" uniqKey="Pierzy Ska Mach A">A. Pierzyńska-Mach</name></author><author><name sortKey="Janowski, P A" uniqKey="Janowski P">P.A. Janowski</name></author><author><name sortKey="Dobrucki, J W" uniqKey="Dobrucki J">J.W. Dobrucki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Repnik, U" uniqKey="Repnik U">U. Repnik</name></author><author><name sortKey="Hafner Esen, M" uniqKey="Hafner Esen M">M. Hafner Česen</name></author><author><name sortKey="Turk, B" uniqKey="Turk B">B. Turk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, W" uniqKey="Song W">W. Song</name></author><author><name sortKey="Popp, L" uniqKey="Popp L">L. Popp</name></author><author><name sortKey="Yang, J" uniqKey="Yang J">J. Yang</name></author><author><name sortKey="Kumar, A" uniqKey="Kumar A">A. Kumar</name></author><author><name sortKey="Gangoli, V S" uniqKey="Gangoli V">V.S. Gangoli</name></author><author><name sortKey="Segatori, L" uniqKey="Segatori L">L. Segatori</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, Y K" uniqKey="Lai Y">Y.-K. Lai</name></author><author><name sortKey="Lee, W C" uniqKey="Lee W">W.-C. Lee</name></author><author><name sortKey="Hu, C H" uniqKey="Hu C">C.-H. Hu</name></author><author><name sortKey="Hammond, G L" uniqKey="Hammond G">G.L. Hammond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shirmohammadi, F" uniqKey="Shirmohammadi F">F. Shirmohammadi</name></author><author><name sortKey="Hasheminassab, S" uniqKey="Hasheminassab S">S. Hasheminassab</name></author><author><name sortKey="Saffari, A" uniqKey="Saffari A">A. Saffari</name></author><author><name sortKey="Schauer, J J" uniqKey="Schauer J">J.J. Schauer</name></author><author><name sortKey="Delfino, R J" uniqKey="Delfino R">R.J. Delfino</name></author><author><name sortKey="Sioutas, C" uniqKey="Sioutas C">C. Sioutas</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region><settlement><li>Los Angeles</li></settlement><orgName><li>Université de Californie du Sud</li></orgName></list><tree><noCountry><name sortKey="Sioutas, Constantinos" sort="Sioutas, Constantinos" uniqKey="Sioutas C" first="Constantinos" last="Sioutas">Constantinos Sioutas</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Sipos, Arnold" sort="Sipos, Arnold" uniqKey="Sipos A" first="Arnold" last="Sipos">Arnold Sipos</name></region><name sortKey="Crandall, Edward D" sort="Crandall, Edward D" uniqKey="Crandall E" first="Edward D." last="Crandall">Edward D. Crandall</name><name sortKey="Crandall, Edward D" sort="Crandall, Edward D" uniqKey="Crandall E" first="Edward D." last="Crandall">Edward D. Crandall</name><name sortKey="Crandall, Edward D" sort="Crandall, Edward D" uniqKey="Crandall E" first="Edward D." last="Crandall">Edward D. Crandall</name><name sortKey="Kim, Kwang Jin" sort="Kim, Kwang Jin" uniqKey="Kim K" first="Kwang-Jin" last="Kim">Kwang-Jin Kim</name><name sortKey="Kim, Kwang Jin" sort="Kim, Kwang Jin" uniqKey="Kim K" first="Kwang-Jin" last="Kim">Kwang-Jin Kim</name><name sortKey="Kim, Kwang Jin" sort="Kim, Kwang Jin" uniqKey="Kim K" first="Kwang-Jin" last="Kim">Kwang-Jin Kim</name><name sortKey="Kim, Kwang Jin" sort="Kim, Kwang Jin" uniqKey="Kim K" first="Kwang-Jin" last="Kim">Kwang-Jin Kim</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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